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KMID : 0363420110250010028
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Journal of Korean Oriental Pediatrics
2011 Volume.25 No. 1 p.28 ~ p.39
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Suppressive Effect of SaengRyoSaMulTang on Activated RBL-2H3 Mast Cells
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Son Mi-Ju
Han Jae-Kyung
Kim Yun-Hee
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Abstract
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Objectives: SaengRyoSaMulTang is a herbal formula in Oriental Medicine, known anti-allergens. However, its mechanism and cellular targets have not been found yet. Thus the study has developed to investigate the suppressive effect of SaengRyoSaMulTang.
Methods: In the study, cellular viability, IL-4, IL-13 mRNA expression, IL-4, IL-13 production, manifestations of GATA-1, GATA-2, NF-AT1, NF-AT2, AP-1 and NF-B p65 transcription factors were examined by Real-Time PCR, ELISA analysis and western blotting.
Results: As a result of treating with SaengRyoSaMulTang extract(SRSMT), the study has shown that the amount of Th2 cytokines, which include PI induced IL-4 and IL-13, plays a significant role in suppressing effect. RBL-2H3 mast cells significantly suppressed the PI-induced Th2 cytokine production including IL-4 and IL-13 in a dose dependent manner. PI-induced IL-4 and IL-13 production was significantly suppressed by SRSMT intervention. Western blot analysis of transcription factors involving IL-4 and IL-13 expression also revealed a prominent decreases of mast cell’s specific transcription factors including GATA-1, GATA-2, NF-AT2, c-Jun and c-Fos, but NF-B p65.
Conclusions: The study suggests that the anti-allergenic activities of SRSMT may regulate the transcription factors GATA-1, GATA-2, NF-AT2, c-Jun and c-Fos inhibiting Th2 cytokines IL-4 and IL-13 in mast cells.
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KEYWORD
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SaengRyoSaMulTang(生料四物湯), RBL-2H3 mast cell, IL-4, IL-13, Anti-allergic effect
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